Optical Imaging Deep within Biological Tissues

Optical characterization and imaging of biological tissues

The biological tissues exhibit their distinct optical characteristics. Their point-to-point compositional variation could either be determined by the optical parameters or reconstruction of the reflectance or tomographic images. The optical parameters, absorption and reduced scattering coefficients of goat tissues and human post-mastectomy breast specimen are determined by matching of their ref...

Microwave Imaging of Biological Tissues

Optical properties of biological tissues: a review.

A review of reported tissue optical properties summarizes the wavelength-dependent behavior of scattering and absorption. Formulae are presented for generating the optical properties of a generic tissue with variable amounts of absorbing chromophores (blood, water, melanin, fat, yellow pigments) and a variable balance between small-scale scatterers and large-scale scatterers in the ultrastructu...

Nipkow confocal imaging from deep brain tissues.

One of the problems in imaging from brain tissues is light-scattering. Thus, multiphoton laser scanning microscopy is widely used to optically access fluorescent signals located deeply in tissues. Here we report that Nipkow-type spinning-disk one-photon confocal microscopy, which embodies high temporal resolution and slow photobleaching, is also capable of imaging tissues to a depth of up to 15...

Microwave-induced acoustic imaging of biological tissues

We present tomographic imaging of biological tissues by use of microwave-induced acoustic signal. It was demonstrated that the acoustic signal was proportional to the intensity of the incident microwave and was related to the absorption property of microwave in the medium. Pulsed microwave radiation was used to illuminate the samples. Absorbed microwave energy caused thermoelastic expansion tha...